Comparison of sarcoplasmic reticulum calcium content in atrial and ventricular myocytes of three fish species
Identifieur interne : 000640 ( Main/Exploration ); précédent : 000639; suivant : 000641Comparison of sarcoplasmic reticulum calcium content in atrial and ventricular myocytes of three fish species
Auteurs : Jaakko Haverinen [Finlande] ; Matti Vornanen [Finlande]Source :
- American journal of physiology. Regulatory, integrative and comparative physiology [ 0363-6119 ] ; 2009.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Calcium.
English descriptors
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Abstract
Ryanodine (Ry) sensitivity of cardiac contraction differs between teleost species, between atrium and ventricle, and according to the thermal history of the fish. The hypothesis that variability in Ry sensitivity of contraction is due to species-specific, chamber-specific, and temperature-related differences in the sarcoplasmic reticulum (SR) Ca2+ content, was tested by comparing steady-state (SS) and maximal (Max) Ca2+ loads of the SR in three teleost fish, rainbow trout (Oncorhynchus mykiss), burbot (Lota lota), and crucian carp (Carassius carassius), which differ in the extent of SR contribution to excitation-contraction coupling. Fish were acclimated at 4°C (cold-acclimation, CA) or 18°C (warm-acclimation, WA), and SR Ca2+ content was released by a rapid application of 10 mM caffeine to single cardiac myocytes; its amount was determined from the Na+-Ca2+ exchange current at 18°C. SS Ca2+ load was larger in atrial (304-915 μmol/l) than ventricular (224-540 μmol/l) myocytes in all fish species (P < 0.05), and the same was true for Max SR Ca2+ content: 550-1,522 μmol/l and 438-840 μmol/l for atrial and ventricular myocytes, respectively (P < 0.05). Consistent with the hypothesis, acclimation to cold increased Ca2+ load of the cardiac SR in the burbot heart, but contrary to the hypothesis, temperature acclimation did not affect SR Ca2+ content in rainbow trout and crucian carp hearts. Furthermore, there was an inverse relation between SR Ca2+ content and Ry sensitivity of contraction force: the species with the smallest SR Ca2+ content (burbot) is most sensitive to Ry. Collectively, these findings show that SR Ca2+ content of fish cardiac myocytes is several times larger than that in mammalian cardiac SR.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Comparison of sarcoplasmic reticulum calcium content in atrial and ventricular myocytes of three fish species</title><author><name sortKey="Haverinen, Jaakko" sort="Haverinen, Jaakko" uniqKey="Haverinen J" first="Jaakko" last="Haverinen">Jaakko Haverinen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Joensuu, Faculty of Biosciences</s1><s2>Joensuu</s2><s3>FIN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Finlande</country><wicri:noRegion>Joensuu</wicri:noRegion></affiliation></author><author><name sortKey="Vornanen, Matti" sort="Vornanen, Matti" uniqKey="Vornanen M" first="Matti" last="Vornanen">Matti Vornanen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Joensuu, Faculty of Biosciences</s1><s2>Joensuu</s2><s3>FIN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Finlande</country><wicri:noRegion>Joensuu</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">09-0420924</idno><date when="2009">2009</date><idno type="stanalyst">PASCAL 09-0420924 INIST</idno><idno type="RBID">Pascal:09-0420924</idno><idno type="wicri:Area/PascalFrancis/Corpus">000037</idno><idno type="wicri:Area/PascalFrancis/Curation">000081</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000038</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000038</idno><idno type="wicri:doubleKey">0363-6119:2009:Haverinen J:comparison:of:sarcoplasmic</idno><idno type="wicri:Area/Main/Merge">000646</idno><idno type="wicri:Area/Main/Curation">000640</idno><idno type="wicri:Area/Main/Exploration">000640</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Comparison of sarcoplasmic reticulum calcium content in atrial and ventricular myocytes of three fish species</title><author><name sortKey="Haverinen, Jaakko" sort="Haverinen, Jaakko" uniqKey="Haverinen J" first="Jaakko" last="Haverinen">Jaakko Haverinen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Joensuu, Faculty of Biosciences</s1><s2>Joensuu</s2><s3>FIN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Finlande</country><wicri:noRegion>Joensuu</wicri:noRegion></affiliation></author><author><name sortKey="Vornanen, Matti" sort="Vornanen, Matti" uniqKey="Vornanen M" first="Matti" last="Vornanen">Matti Vornanen</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>University of Joensuu, Faculty of Biosciences</s1><s2>Joensuu</s2><s3>FIN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Finlande</country><wicri:noRegion>Joensuu</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">American journal of physiology. Regulatory, integrative and comparative physiology</title><title level="j" type="abbreviated">Am. j. physiol., Regul. integr. comp. physiol.</title><idno type="ISSN">0363-6119</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">American journal of physiology. Regulatory, integrative and comparative physiology</title><title level="j" type="abbreviated">Am. j. physiol., Regul. integr. comp. physiol.</title><idno type="ISSN">0363-6119</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Atrium</term><term>Calcium</term><term>Carassius carassius</term><term>Carp</term><term>Load</term><term>Myocyte</term><term>Oncorhynchus mykiss</term><term>Ryanodine</term><term>Sarcoplasmic reticulum</term><term>Sensitivity</term><term>Steady state</term><term>Temperature</term><term>Trout</term><term>Variability</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Réticulum sarcoplasmique</term><term>Calcium</term><term>Myocyte</term><term>Ryanodine</term><term>Sensibilité</term><term>Oreillette</term><term>Variabilité</term><term>Température</term><term>Régime permanent</term><term>Charge</term><term>Truite</term><term>Carpe</term><term>Oncorhynchus mykiss</term><term>Carassius carassius</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Calcium</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ryanodine (Ry) sensitivity of cardiac contraction differs between teleost species, between atrium and ventricle, and according to the thermal history of the fish. The hypothesis that variability in Ry sensitivity of contraction is due to species-specific, chamber-specific, and temperature-related differences in the sarcoplasmic reticulum (SR) Ca<sup>2+</sup> content, was tested by comparing steady-state (SS) and maximal (Max) Ca<sup>2+</sup> loads of the SR in three teleost fish, rainbow trout (Oncorhynchus mykiss), burbot (Lota lota), and crucian carp (Carassius carassius), which differ in the extent of SR contribution to excitation-contraction coupling. Fish were acclimated at 4°C (cold-acclimation, CA) or 18°C (warm-acclimation, WA), and SR Ca<sup>2+</sup> content was released by a rapid application of 10 mM caffeine to single cardiac myocytes; its amount was determined from the Na<sup>+</sup>-Ca<sup>2+</sup> exchange current at 18°C. SS Ca<sup>2+</sup> load was larger in atrial (304-915 μmol/l) than ventricular (224-540 μmol/l) myocytes in all fish species (P < 0.05), and the same was true for Max SR Ca<sup>2+</sup> content: 550-1,522 μmol/l and 438-840 μmol/l for atrial and ventricular myocytes, respectively (P < 0.05). Consistent with the hypothesis, acclimation to cold increased Ca<sup>2+</sup> load of the cardiac SR in the burbot heart, but contrary to the hypothesis, temperature acclimation did not affect SR Ca<sup>2+</sup> content in rainbow trout and crucian carp hearts. Furthermore, there was an inverse relation between SR Ca<sup>2+</sup> content and Ry sensitivity of contraction force: the species with the smallest SR Ca<sup>2+</sup> content (burbot) is most sensitive to Ry. Collectively, these findings show that SR Ca<sup>2+</sup> content of fish cardiac myocytes is several times larger than that in mammalian cardiac SR.</div></front></TEI><affiliations><list><country><li>Finlande</li></country></list><tree><country name="Finlande"><noRegion><name sortKey="Haverinen, Jaakko" sort="Haverinen, Jaakko" uniqKey="Haverinen J" first="Jaakko" last="Haverinen">Jaakko Haverinen</name></noRegion><name sortKey="Vornanen, Matti" sort="Vornanen, Matti" uniqKey="Vornanen M" first="Matti" last="Vornanen">Matti Vornanen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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